Rapid Characterization of Cellular Pathways Using Time-Varying Signals
Name
poster v1.ppt
Size
2.64 MB
Format
Microsoft Powerpoint
Checksum (MD5)
90b24dfc7dd882326c8fe155ca06642b
Author(s)
Thomson, Ty M
Endy, Drew
Date Issued
October 21, 2005
Publisher
International Conference on Systems Biology
Citation
Ty Thomson and Drew Endy. Rapid Characterization of Cellular Pathways Using Time-Varying Signals. Sixth International Conference on Systems Biology, 2005.
Abstract
The use of traditional tools for the discovery and characterization of biological systems has resulted in a wealth of biological knowledge. Unfortunately, only a small portion of the biological world is well-understood to date, and the study of the rest remains a daunting task. This work involves using time-varying stimuli in order to more rapidly interrogate and characterize signaling pathways. The time-dependent stimulation of a signaling pathway can be used in conjunction with a model of the pathway to efficiently evaluate and test hypotheses. We are developing this technology using the yeast pheromone signal transduction pathway as a model system. The time-varying stimuli will be applied to the yeast cells via a novel microfluidic device, and the pathway output will be measured via various fluorescent reporters. The output of the pathway can then be compared to the output from a computational model of the pathway in order to test hypotheses and constrain our knowledge of the pathway. Initial work shows that a computational model can be used to identify stimuli time-courses that increase the parameter sensitivity, meaning that corresponding experiments could potentially be much more informative.
Description
Poster presented at the 2005 ICSB meeting, held at Harvard Medical School in Boston, MA.
Subjects
Pheromone Response
Time-Varying Signals
Microfluidics
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Persistent DSpace Link
