Retroactivity, modularity, and insulation in synthetic biology circuits
Name
825553696-MIT.pdf
Description
Full printable version
Size
18.84 MB
Format
Adobe PDF
Checksum (MD5)
89099018d27e400e8112790f446e00cd
Author(s)
Lin, Allen
Advisor(s)
Ron Weiss and Domitilla Del Vecchio.
Alternative Title
Modularity, Retroactivity, and insulation in synthetic biology circuits
Date Issued
2011
Publisher
Massachusetts Institute of Technology
Abstract
A central concept in synthetic biology is the reuse of well-characterized modules. Modularity simplifies circuit design by allowing for the decomposition of systems into separate modules for individual construction. Complex regulatory networks can be assembled from a library of devices. However, current devices in synthetic biology may not actually be modular and may instead change behavior upon interconnections, a phenomenon called retroactivity. Addition of a new component to a system can change individual device dynamics within the system, potentially making timeconsuming iterative redesign necessary. Another need for systems construction is the ability to rapidly assemble constructs from part libraries in a combinatorial, highthroughput fashion. In this thesis, a multi-site assembly method that permits the rapid reshuffling of promoters and genes for yeast expression is established. Synthetic circuits in yeast to measure retroactivity and to act as an insulator that attenuates such effect are designed and modeled.
Description
Thesis (M. Eng.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 2011.
Cataloged from PDF version of thesis.
Includes bibliographical references (p. 141-151).
Subjects
Electrical Engineering and Computer Science.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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