Computational identification and experimental validation of PPRE motifs in NHE1 and MnSOD genes of Human
Name
Venkatachalam-2009-Computational identi.pdf
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Author(s)
Venkatachalam, Gireedhar
Kumar, Alan Prem
Yue, Loo Ser
Pervaiz, Shazib
Clement, Marie Veronique
Sakharkar, Meena Kishore
Date Issued
December 2009
Journal
BMC Genomics
Publisher
BioMed Central
Citation
Venkatachalam, Gireedhar et al. “Computational Identification and Experimental Validation of PPRE Motifs in NHE1 and MnSOD Genes of Human.” BMC Genomics 10.Suppl 3 (2009) : S5. © 2009 Venkatachalam et al; licensee BioMed Central Ltd.
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Final published version
Abstract
Background: Activation of PPARs has been reported to inhibit the proliferation of malignant
cells from different lineages. They are involved in transcription regulation of genes upon activation
by a ligand. The binding of PPARs to the promoter sequence either represses or activates the gene.
Hence, PPARs represent promising targets for cancer treatment because of their anti-proliferative
and pro-apoptotic activities. Here we computationally identified PPAR binding regions in NHE1 and
MnSOD. We further validated the predictions in vitro.
Results: Our results computationally predicted the presence of 2 PPRE motifs in NHE1 and 3
PPRE motifs in MnSOD. We experimentally confirmed the true motifs and their regulation by
PPAR.
Conclusion: Our results suggest that both NHE1 and MnSOD have PPRE binding motif in their
upstream/promoter region and hence are regulated by PPAR upon ligand binding.
MIT Department
Singapore-MIT Alliance in Research and Technology (SMART)
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DOI of Published Version
https://doi.org/10.1186/1471-2164-10-s3-s5
