Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters
Name
Imperiali_Monitoring protein.pdf
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1.69 MB
Format
Adobe PDF
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Author(s)
Lukovic, Elvedin
Taylor, Elizabeth Vogel
Imperiali, Barbara
Date Issued
August 2009
Journal
Angewandte Chemie International Edition
Publisher
Wiley Blackwell (John Wiley & Sons)
Citation
Luković, Elvedin, Elizabeth Vogel Taylor, and Barbara Imperiali. “Monitoring Protein Kinases in Cellular Media with Highly Selective Chimeric Reporters.” Angewandte Chemie International Edition 48.37 (2009): 6828–6831.
Version
Author's final manuscript
Abstract
The best of both worlds: Combining a recombinant docking domain (see picture, blue ribbon) with a chemical sensing module (green hexagons) yields a highly selective ERK sensor. This probe exclusively monitors ERK1/2 activity in unfractionated cell lysates without any off-target kinase inhibitors. Good selectivity and biophysical parameters allow high-throughput analysis of ERK1/2 activity without time-consuming enzyme purification.
Description
2010 September 13
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1002/anie.200902374
