Three-dimensional cardiac architecture determined by two-photon microtomy
Name
Huang-2009-Three-dimensional ca.pdf
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1.89 MB
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Checksum (MD5)
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Author(s)
Huang, Hayden
MacGillivray, Catherine
So, Peter T. C.
Kwon, Hyuk-Sang
Lammerding, Jan
Robbins, Jeffrey
Lee, Richard T.
Date Issued
August 2009
Journal
Journal of Biomedical Optics
Publisher
Society of Photo-Optical Instrumentation Engineers
Citation
Huang, Hayden et al. “Three-dimensional cardiac architecture determined by two-photon microtomy.” Journal of Biomedical Optics 14.4 (2009): 044029-10. © 2009 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
Cardiac architecture is inherently three-dimensional, yet most characterizations rely on two-dimensional histological slices or dissociated cells, which remove the native geometry of the heart. We previously developed a method for labeling intact heart sections without dissociation and imaging large volumes while preserving their three-dimensional structure. We further refine this method to permit quantitative analysis of imaged sections. After data acquisition, these sections are assembled using image-processing tools, and qualitative and quantitative information is extracted. By examining the reconstructed cardiac blocks, one can observe end-to-end adjacent cardiac myocytes (cardiac strands) changing cross-sectional geometries, merging and separating from other strands. Quantitatively, representative cross-sectional areas typically used for determining hypertrophy omit the three-dimensional component; we show that taking orientation into account can significantly alter the analysis. Using fast-Fourier transform analysis, we analyze the gross organization of cardiac strands in three dimensions. By characterizing cardiac structure in three dimensions, we are able to determine that the alpha crystallin mutation leads to hypertrophy with cross-sectional area increases, but not necessarily via changes in fiber orientation distribution.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
http://dx.doi.org/10.1117/1.3200939
