The nature of cell division forces in epithelial monolayers
Name
jcb_202011106.pdf
Description
Published version
Size
7.62 MB
Format
Adobe PDF
Checksum (MD5)
354c0978150fcbe808f44e24355073fc
Author(s)
Gupta, Vivek K
Nam, Sungmin
Yim, Donghyun
Camuglia, Jaclyn
Martin, Judy Lisette
Sanders, Erin Nicole
O’Brien, Lucy Erin
Martin, Adam C
Kim, Taeyoon
Chaudhuri, Ovijit
Date Issued
2021
Journal
The Journal of Cell Biology
Publisher
Rockefeller University Press
Version
Final published version
Abstract
Epithelial cells undergo striking morphological changes during division to ensure proper segregation of genetic and cytoplasmic materials. These morphological changes occur despite dividing cells being mechanically restricted by neighboring cells, indicating the need for extracellular force generation. Beyond driving cell division itself, forces associated with division have been implicated in tissue-scale processes, including development, tissue growth, migration, and epidermal stratification. While forces generated by mitotic rounding are well understood, forces generated after rounding remain unknown. Here, we identify two distinct stages of division force generation that follow rounding: (1) Protrusive forces along the division axis that drive division elongation, and (2) outward forces that facilitate postdivision spreading. Cytokinetic ring contraction of the dividing cell, but not activity of neighboring cells, generates extracellular forces that propel division elongation and contribute to chromosome segregation. Forces from division elongation are observed in epithelia across many model organisms. Thus, division elongation forces represent a universal mechanism that powers cell division in confining epithelia.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1083/jcb.202011106
