Temperature dependence of ssrA-tag mediated protein degradation
Name
1754-1611-6-10.pdf
Size
566.23 KB
Format
Adobe PDF
Checksum (MD5)
62a3a7174919b588b714b26eb0d15b37
Author(s)
Purcell, Oliver
Grierson, Claire S
Bernardo, Mario
Savery, Nigel J
Date Issued
July 2012
Journal
Journal of Biological Engineering
Publisher
BioMed Central Ltd.
Citation
Purcell, Oliver et al. “Temperature Dependence of ssrA-tag Mediated Protein Degradation.” Journal of Biological Engineering 6.1 (2012): 10. Web.
Version
Final published version
Abstract
Building synthetic gene networks with highly transient dynamics requires rapid protein degradation. We show that the degradation conferred by two commonly used ssrA tags is highly temperature dependent. Synthetic gene networks are being used increasingly in real-world applications where they may be subjected to variable conditions, and be required to display precise, quantitative dynamics, which will be more susceptible to environmental changes than the general qualitative dynamics focussed on so far.
MIT Department
Massachusetts Institute of Technology. Research Laboratory of Electronics
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/1754-1611-6-10
