Architecture and evolutionary stability of yeast signaling pathways
Name
85844464-MIT.pdf
Description
Full printable version
Size
1.94 MB
Format
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Checksum (MD5)
ebe57bd93d757e7e35f10419599919b8
Author(s)
Gritton, Jeffrey S
Advisor(s)
Drew Endy.
Date Issued
2006
Publisher
Massachusetts Institute of Technology
Abstract
I have researched the effect that selection for the function of the High Osmolarity Glycerol (HOG) pathway has on the evolutionary stability of the pheromone response pathway in the yeast Saccharomyces cerevisiae. I first set out to demonstrate that, because the pheromone and HOG response pathways share protein components, selection for HOG function will enrich a population for cells capable of pheromone response. I performed experiments in both continuous and batch culture to demonstrate this effect. I then characterized the decay of the pheromone response pathway first, by measuring its mutation rate and second, by measuring the fitness of a series of strains with pheromone response gene deletions. I conclude with thoughts on possible experiments that may be used to further this research.
Description
Thesis (S.M.)--Massachusetts Institute of Technology, Dept. of Biology, 2006.
"September 2006."
Includes bibliographical references (leaves 37-38).
Subjects
Biology.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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