Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy
Name
Sapsis_Circadian rhythms.pdf
Size
1.47 MB
Format
Adobe PDF
Checksum (MD5)
12213e9ca2c8821f5ba670ddde3cd7aa
Author(s)
Petsakou, Afroditi
Blau, Justin
Sapsis, Themistoklis P.
Date Issued
July 2015
Journal
Cell
Publisher
Elsevier
Citation
Petsakou, Afroditi, Themistoklis P. Sapsis, and Justin Blau. “Circadian Rhythms in Rho1 Activity Regulate Neuronal Plasticity and Network Hierarchy.” Cell 162.4 (2015): 823–835. © 2017 Elsevier
Version
Final published version
Abstract
Neuronal plasticity helps animals learn from their environment. However, it is challenging to link specific changes in defined neurons to altered behavior. Here, we focus on circadian rhythms in the structure of the principal s-LNv clock neurons in Drosophila. By quantifying neuronal architecture, we observed that s-LNv structural plasticity changes the amount of axonal material in addition to cycles of fasciculation and defasciculation. We found that this is controlled by rhythmic Rho1 activity that retracts s-LNv axonal termini by increasing myosin phosphorylation and simultaneously changes the balance of pre-synaptic and dendritic markers. This plasticity is required to change clock network hierarchy and allow seasonal adaptation. Rhythms in Rho1 activity are controlled by clock-regulated transcription of Puratrophin-1-like (Pura), a Rho1 GEF. Since spinocerebellar ataxia is associated with mutations in human Puratrophin-1, our data support the idea that defective actin-related plasticity underlies this ataxia.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.cell.2015.07.010
