Negative Regulation of Fibroblast Motility by Ena/VASP Proteins
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Gertler_Negative regulation.pdf
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Author(s)
Libova, Irina
Bear, James E.
Loureiro, Joseph J.
Gertler, Frank
Fassler, Reinhard
Wehland, Jurgen
Date Issued
June 2000
Journal
Cell
Publisher
Elsevier
Citation
Bear, James E, Joseph J Loureiro, Irina Libova, Reinhard Fassler, Jurgen Wehland, and Frank B Gertler. “Negative Regulation of Fibroblast Motility by Ena/VASP Proteins.” Cell 101, no. 7 (June 2000): 717-728. Copyright © 2000 Cell Press
Version
Final published version
Abstract
Ena/VASP proteins have been implicated in cell motility through regulation of the actin cytoskeleton and are found at focal adhesions and the leading edge. Using overexpression, loss-of-function, and inhibitory approaches, we find that Ena/VASP proteins negatively regulate fibroblast motility. A dose-dependent decrease in movement is observed when Ena/VASP proteins are overexpressed in fibroblasts. Neutralization or deletion of all Ena/VASP proteins results in increased cell movement. Selective depletion of Ena/VASP proteins from focal adhesions, but not the leading edge, has no effect on motility. Constitutive membrane targeting of Ena/VASP proteins inhibits motility. These results are in marked contrast to current models for Ena/VASP function derived mainly from their role in the actin-driven movement of Listeria monocytogenes.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/S0092-8674(00)80884-3
