High-Throughput Nonlinear Optical Microscopy
Name
So-2013-High-Throughput Nonl.pdf
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2.59 MB
Format
Adobe PDF
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Author(s)
Yew, Elijah Y.S.
So, Peter T. C.
Rowlands, Christopher
Yew, Yan Seng Elijah
Date Issued
December 2013
Journal
Biophysical Journal
Publisher
Elsevier
Citation
So, Peter T.C., Elijah Y.S. Yew, and Christopher Rowlands. “High-Throughput Nonlinear Optical Microscopy.” Biophysical Journal 105, no. 12 (December 2013): 2641–2654.
Version
Final published version
Abstract
High-resolution microscopy methods based on different nonlinear optical (NLO) contrast mechanisms are finding numerous applications in biology and medicine. While the basic implementations of these microscopy methods are relatively mature, an important direction of continuing technological innovation lies in improving the throughput of these systems. Throughput improvement is expected to be important for studying fast kinetic processes, for enabling clinical diagnosis and treatment, and for extending the field of image informatics. This review will provide an overview of the fundamental limitations on NLO microscopy throughput. We will further cover several important classes of high-throughput NLO microscope designs with discussions on their strengths and weaknesses and their key biomedical applications. Finally, this review will close with a perspective of potential future technological improvements in this field.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Laser Biomedical Research Center
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1016/j.bpj.2013.08.051
