Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis
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Hopkins_Essential genes.pdf
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1.29 MB
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Author(s)
Barresi, Michael J. F.
Burton, Sean
Dipietrantonio, Kristina
Amsterdam, Adam
Hopkins, Nancy H.
Karlstrom, Rolf O.
Date Issued
August 2010
Journal
Developmental Dynamics
Publisher
Wiley Blackwell (John Wiley & Sons)
Citation
Barresi, Michael J.F. et al. “Essential Genes for Astroglial Development and Axon Pathfinding During Zebrafish Embryogenesis.” Developmental Dynamics 239.10 (2010): 2603–2618.
Version
Author's final manuscript
Abstract
The formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a “shelf-screen” to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti-Acetylated Tubulin) and astroglia (anti-Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon-glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio- and neurogenesis, and neuron-glial interactions during development of the vertebrate CNS.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1002/dvdy.22393
