Monitoring protein interactions and dynamics with solvatochromic fluorophores
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Imperiali6_Loving_TIB_2009.pdf
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4.77 MB
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1203e7b0e209bb2e45bfb854ab4063cf
Author(s)
Loving, Galen S.
Sainlos, Matthieu
Imperiali, Barbara
Date Issued
February 2010
Journal
Trends in Biotechnology
Publisher
Elsevier B.V.
Citation
Loving, Galen S., Matthieu Sainlos, and Barbara Imperiali. “Monitoring Protein Interactions and Dynamics with Solvatochromic Fluorophores.” Trends in Biotechnology 28.2 (2010): 73–83.
Version
Author's final manuscript
Abstract
Solvatochromic fluorophores possess emission properties that are sensitive to the nature of the local microenvironment. These dyes have been exploited in applications ranging from the study of protein structural dynamics to the detection of protein-binding interactions. Although the solvatochromic indole fluorophore of tryptophan has been utilized extensively for in vitro studies to advance our understanding of basic protein biochemistry, the emergence of new extrinsic synthetic dyes with improved properties, in conjunction with recent developments in site-selective methods to incorporate these chemical tools into proteins, now open the way for studies in more complex systems. Herein, we discuss recent technological advancements and their application in the design of powerful reporters, which serve critical roles in modern cell biology and assay development.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1016/j.tibtech.2009.11.002
