Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay
Name
Lee JACS Comm subm 2009.pdf
Size
874.75 KB
Format
Adobe PDF
Checksum (MD5)
b551cf3a125e394885bc32743fa175c7
Author(s)
Lee, Jeong Hoon
Cosgrove, Benjamin D.
Lauffenburger, Douglas A.
Han, Jongyoon
Date Issued
July 2009
Journal
Journal of the American Chemical Society
Publisher
American Chemical Society
Citation
Lee, Jeong Hoon et al. “Microfluidic Concentration-Enhanced Cellular Kinase Activity Assay.” Journal of the American Chemical Society 131.30 (2009): 10340-10341.
Version
Author's final manuscript
Abstract
In this paper, we reported a simple, disposable PDMS micro/nanofluidic preconcentration chip for in vitro concentration-enhanced cell kinase assays. Utilizing the preconcentration (electrokinetic trapping) directly from cell lysate (1 mM ATP) samples, we could achieve at least a 25-fold increase in reaction velocity and 65-fold enhancement in sensitivity. In addition, we shorten the assay time down to less than 10 min, with the sample volume requirements of down to 5 cells. This device could be a generic and powerful tool for diagnostics and systems biology studies at the single-cell level, if properly optimized and integrated with the cell culture microdevices.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Attribution-Noncommercial-Share Alike 3.0 Unported
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1021/ja902594f
