Dynamic deformability of Plasmodium falciparum-infected erythrocytes exposed to artesunate in vitro
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Han_Dynamic deformability.pdf
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Author(s)
Huang, Sha
Undisz, Andreas
Diez Silva, Monica
Bow, Hansen
Dao, Ming
Han, Jongyoon
Date Issued
November 2012
Journal
Integrative Biology
Publisher
Royal Society of Chemistry
Citation
Huang, Sha, Andreas Undisz, Monica Diez-Silva, Hansen Bow, Ming Dao, and Jongyoon Han. “Dynamic Deformability of Plasmodium Falciparum-Infected Erythrocytes Exposed to Artesunate in Vitro.” Integr. Biol. 5, no. 2 (2013): 414.
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Author's final manuscript
Abstract
Artesunate (ART) is widely used for the treatment of malaria, but the mechanisms of its effects on parasitized red blood cells (RBCs) are not fully understood. We investigated ART's influence on the dynamic deformability of ring-stage Plasmodium falciparum infected red blood cells (iRBCs) in order to elucidate its role in cellular mechanobiology. The dynamic deformability of RBCs was measured by passing them through a microfluidic device with repeated bottleneck structures. The quasi-static deformability measurement was performed using micropipette aspiration. After ART treatment, microfluidic experiments showed 50% decrease in iRBC transit velocity whereas only small (~10%) velocity reduction was observed among uninfected RBCs (uRBCs). Micropipette aspiration also revealed ART-induced stiffening in RBC membranes. These results demonstrate, for the first time, that ART reduces the dynamic and quasi-static RBC deformability, which may subsequently influence blood circulation through the microvasculature and spleen cordal meshwork, thus adding a new aspect to artesunate's mechanism of action.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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DOI of Published Version
https://doi.org/10.1039/c2ib20161e
