Antibodies and methods for immunohistochemistry of extracellular matrix proteins
Name
Rickelt and Hynes 2018-1.pdf
Description
Accepted version
Size
44.23 MB
Format
Adobe PDF
Checksum (MD5)
aa59d2e6d25a7f2f50535f700fe0b1ef
Author(s)
Rickelt, Steffen
Hynes, Richard O
Date Issued
October 2018
Journal
Matrix Biology
Publisher
Elsevier BV
Citation
Rickelt, Steffen and Richard O. Hynes. "Antibodies and methods for immunohistochemistry of extracellular matrix proteins." 71-72 (October 2018): 10-27. © 2018 Elsevier B.V.
Version
Author's final manuscript
Abstract
The diversity of extracellular matrix (ECM) proteins encoded in mammalian genomes and detected by proteomic analyses generates a need for well validated antibodies against these proteins. We present characterization of a large number of antibodies against ECM proteins, from both commercial and academic sources, together with discussion of methods and strategies for their effective use in immunohistochemistry and illustrations of their efficacy. These data should be of value to investigators seeking well validated antibodies to ECM proteins of interest and save significant time and money tracking down effective reagents.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.matbio.2018.04.011
