Modeling the Electrophoretic Separation of Short Biological Molecules in Nanofluidic Devices
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Modeling the electrophoretic.pdf
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Author(s)
Fayad, Ghassan
Hadjiconstantinou, Nicolas
Date Issued
March 2013
Journal
Journal of Fluids Engineering
Publisher
ASME International
Citation
Fayad, Ghassan N. and Hadjiconstantinou, Nicolas G. “Modeling the Electrophoretic Separation of Short Biological Molecules in Nanofluidic Devices.” Journal of Fluids Engineering 135, no. 2 (March 2013): 024501. © 2013 ASME
Version
Final published version
Abstract
Via comparisons with rigid-rod and wormlike-chain Brownian dynamics (BD) simulations and the experimental results of Fu et al. (2006, “Molecular Sieving in Periodic Free-Energy Landscapes Created by Patterned Nanofilter Arrays,” Phys. Rev. Lett., 97(1), p. 018103), we demonstrate that, for the purposes of low-to-medium field electrophoretic separation, sufficiently short biomolecules can be modeled as point particles, with their orientational degrees of freedom accounted for using partition coefficients. This observation is used in the present work to build an efficient BD simulation method. Particular attention is paid to the model's ability to quantitatively capture experimental results using realistic values of all physical parameters.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1115/1.4023445
