The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
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elife-72841-v2.pdf
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Published version
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5.94 MB
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Author(s)
Sauvola, Chad W
Akbergenova, Yulia
Cunningham, Karen L
Aponte-Santiago, Nicole A
Littleton, J Troy
Date Issued
2021
Journal
eLife
Publisher
eLife Sciences Publications, Ltd
Citation
Sauvola, Chad W, Akbergenova, Yulia, Cunningham, Karen L, Aponte-Santiago, Nicole A and Littleton, J Troy. 2021. "The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity." eLife, 10.
Version
Final published version
Abstract
Synaptic vesicle (SV) release probability (Pr) is a key presynaptic determinant of synaptic strength established by cell-intrinsic properties and further refined by plasticity. To characterize mechanisms that generate Pr heterogeneity between distinct neuronal populations, we examined glutamatergic tonic (Ib) and phasic (Is) motoneurons in Drosophila with stereotyped differences in Pr and synaptic plasticity. We found the decoy soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) Tomosyn is differentially expressed between these motoneuron subclasses and contributes to intrinsic differences in their synaptic output. Tomosyn expression enables tonic release in Ib motoneurons by reducing SNARE complex formation and suppressing Pr to generate decreased levels of SV fusion and enhanced resistance to synaptic fatigue. In contrast, phasic release dominates when Tomosyn expression is low, enabling high intrinsic Pr at Is terminals at the expense of sustained release and robust presynaptic potentiation. In addition, loss of Tomosyn disrupts the ability of tonic synapses to undergo presynaptic homeostatic potentiation.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.7554/ELIFE.72841
