Assembly of BioBrick standard biological parts using three antibiotic assembly
Name
BioBrickAssemblyFinalAuthorsVersion.pdf
Description
Revised author's version, compiled
Size
600.77 KB
Format
Adobe PDF
Checksum (MD5)
9f069485b40f343367cbeaff78fcf34d
Author(s)
Shetty, Reshma
Lizarazo, Meagan
Rettberg, Randy
Knight, Thomas F., Jr.
Date Issued
May 20, 2011
Publisher
Elsevier Inc.
Citation
Shetty R, Lizarazo M, Rettberg R, Knight TF. Assembly of BioBrick standard biological parts using three antibiotic assembly. Methods Enzymol. 2011;498:311-26.
Abstract
An underlying goal of synthetic biology is to make the process of engineering biological systems easier and more reliable. In support of this goal, we developed BioBrick assembly standard 10 to enable the construction of systems from standardized genetic parts. The BioBrick standard underpins the distributed efforts by the synthetic biology research community to develop a collection of more than 6000 standard genetic parts available from the Registry of Standard Biological Parts. Here, we describe the three antibiotic assembly method for physical composition of BioBrick parts and provide step-by-step protocols. The method relies on a combination of positive and negative selection to eliminate time- and labor-intensive steps such as column cleanup and agarose gel purification of DNA during part assembly.
Description
This is a revised personal version of the text of the final journal article available via DOI: 10.1016/B978-0-12-385120-8.00013-9
Subjects
biobrick parts
DNA assembly
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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