Elucidation of Beta-Oxidation Pathways in Ralstonia Eutropha H16 by Examination of Global Gene Expression
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Author(s)
Zeng, Qiandong
Holder, Jason W.
Mahan, Alison E.
Brigham, Christopher J.
Budde, Charles F.
Rha, Chokyun
Sinskey, Anthony J
Date Issued
August 2010
Journal
Journal of Bacteriology
Publisher
American Society for Microbiology
Citation
Brigham, C. J. et al. “Elucidation of -Oxidation Pathways in Ralstonia eutropha H16 by Examination of Global Gene Expression.” Journal of Bacteriology 192 (2010): 5454-5464. Web. 4 Nov. 2011. © 2010 American Society for Microbiology
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Author's final manuscript
Abstract
Ralstonia eutropha H16 is capable of growth and polyhydroxyalkanoate production on plant oils and fatty acids. However, little is known about the triacylglycerol and fatty acid degradation pathways of this bacterium. We compare whole-cell gene expression levels of R. eutropha H16 during growth and polyhydroxyalkanoate production on trioleate and fructose. Trioleate is a triacylglycerol that serves as a model for plant oils. Among the genes of note, two potential fatty acid β-oxidation operons and two putative lipase genes were shown to be upregulated in trioleate cultures. The genes of the glyoxylate bypass also exhibit increased expression during growth on trioleate. We observed that single β-oxidation operon deletion mutants of R. eutropha could grow using palm oil or crude palm kernel oil as the sole carbon source, regardless of which operon was present in the genome, but a double mutant was unable to grow under these conditions. A lipase deletion mutant did not exhibit a growth defect in emulsified oil cultures but did exhibit a phenotype in cultures containing nonemulsified oil. Mutants of the glyoxylate shunt gene for isocitrate lyase were able to grow in the presence of oils, while a malate synthase (aceB) deletion mutant grew more slowly than wild type. Gene expression under polyhydroxyalkanoate storage conditions was also examined. Many findings of this analysis confirm results from previous studies by our group and others. This work represents the first examination of global gene expression involving triacylglycerol and fatty acid catabolism genes in R. eutropha.
MIT Department
Massachusetts Institute of Technology. Biomaterials Science and Engineering Laboratory
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Engineering Systems Division
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DOI of Published Version
https://doi.org/10.1128/jb.00493-10
