Size homeostasis in adherent cells studied by synthetic phase microscopy
Name
Sung-2013-Size homeostasis in.pdf
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Author(s)
Sung, Yongjin
Tzur, Amit
Oh, Seungeun
Choi, Wonshik
Li, Victor
Dasari, Ramachandra Rao
Yaqoob, Zahid
Kirschner, Marc W.
Date Issued
October 2013
Journal
Proceedings of the National Academy of Sciences
Publisher
National Academy of Sciences (U.S.)
Citation
Sung, Y., A. Tzur, S. Oh, W. Choi, V. Li, R. R. Dasari, Z. Yaqoob, and M. W. Kirschner. “Size Homeostasis in Adherent Cells Studied by Synthetic Phase Microscopy.” Proceedings of the National Academy of Sciences 110, no. 41 (October 8, 2013): 16687–16692.
Version
Final published version
Abstract
The coupling of the rate of cell growth to the rate of cell division determines cell size, a defining characteristic that is central to cell function and, ultimately, to tissue architecture. The physiology of size homeostasis has fascinated generations of biologists, but the mechanism, challenged by experimental limitations, remains largely unknown. In this paper, we propose a unique optical method that can measure the dry mass of thick live cells as accurately as that for thin cells with high computational efficiency. With this technique, we quantify, with unprecedented accuracy, the asymmetry of division in lymphoblasts and epithelial cells. We can then use the Collins–Richmond model of conservation to compute the relationship between growth rate and cell mass. In attached epithelial cells, we find that due to the asymmetry in cell division and size-dependent growth rate, there is active regulation of cell size. Thus, like nonadherent cells, size homeostasis requires feedback control.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Spectroscopy Laboratory
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1073/pnas.1315290110
