Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation
Name
Kim-2009-Three-Dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation.pdf
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681.76 KB
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06ca9719ed956c1046002d26e4e509c3
Author(s)
Kim, Daekeun
So, Peter T. C.
Date Issued
February 2009
Journal
Proceedings of SPIE--the International Society for Optical Engineering
Publisher
Society of Photo-optical Instrumentation Engineers
Citation
Kim, Daekeun, and Peter T. C. So. “Three-dimensional (3D) high-speed imaging and fabrication system based on ultrafast optical pulse manipulation.” Multiphoton Microscopy in the Biomedical Sciences IX. Ed. Ammasi Periasamy & Peter T. C. So. San Jose, CA, USA: SPIE, 2009. 71831B-8. © 2009 Society of Photo-optical Instrumentation Engineers.
Version
Final published version
Abstract
Laser scanning systems for two-photon microscopy and fabrication have been proven to be excellent in depth-resolving capability for years. However, their applications have been limited to laboratory use due to their intrinsic slow nature. The recently introduced temporal focusing concept enables wide-field optical sectioning and thus has potential in both high-speed 3D imaging and 3D mass-production fields. In this paper, we use the ultrafast optical pulse manipulation to generate two-photon excitation depth-resolved wide-field illumination (TPEDRWFI). The design parameters for the illumination were chosen based on numerical simulation of the temporal focusing. The imaging system was implemented, and the optical sectioning performance was compared with experimental result.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
http://dx.doi.org/10.1117/12.810910
