Strain-Specific Regulation of Striatal Phenotype in Drd2-eGFP BAC Transgenic Mice
Name
9124.full.pdf
Description
Published version
Size
1.74 MB
Format
Unknown
Checksum (MD5)
60826444009552da8aecf113ca3b37cb
Author(s)
Chan, C. S.
Peterson, J. D.
Gertler, T. S.
Glajch, K. E.
Quintana, R. E.
Cui, Q.
Sebel, L. E.
Plotkin, J. L.
Shen, W.
Heiman, M.
Date Issued
July 2012
Journal
Journal of Neuroscience
Publisher
Society for Neuroscience
Citation
Chan, C. S., et al. "Strain-Specific Regulation of Striatal Phenotype in Drd2-Egfp Bac Transgenic Mice." J Neurosci 32 27 (2012): 9124-32.
Version
Final published version
Abstract
Mice carrying bacterial artificial chromosome (BAC) transgenes have become important tools for neuroscientists, providing a powerful means of dissecting complex neural circuits in the brain. Recently, it was reported that one popular line of these mice-mice possessing a BAC transgene with a D 2 dopamine receptor (Drd2) promoter construct coupled to an enhanced green fluorescent protein (eGFP) reporter- had abnormal striatal gene expression, physiology, and motor behavior. Unlike most of the work using BAC mice, this interesting study relied upon mice backcrossed on the outbred Swiss Webster (SW) strain that were homozygous for the Drd2-eGFP BAC transgene. The experiments reported here were conducted to determine whether mouse strain or zygosity was a factor in the reported abnormalities. As reported, SW mice were very sensitive to transgene expression. However, in more commonly used inbred strains of mice (C57BL/6, FVB/N) that were hemizygous for the transgene, the Drd2-eGFP BAC transgene did not alter striatal gene expression, physiology, or motor behavior. Thus, the use of inbred strains of mice that are hemizygous for the Drd2 BAC transgene provides a reliable tool for studying basal ganglia function. © 2012 the authors.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Picower Institute for Learning and Memory
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1523/jneurosci.0229-12.2012
