Accelerating Diverse Cell-Based Therapies Through Scalable Design
Name
annurev-chembioeng-100722-121610.pdf
Description
Published version
Size
1.41 MB
Format
Adobe PDF
Checksum (MD5)
bd51a92e8f37d972a465fda6b3e480b8
Author(s)
Peterman, Emma L
Ploessl, Deon S
Galloway, Kate E
Date Issued
July 24, 2024
Journal
Annual Review of Chemical and Biomolecular Engineering
Publisher
Annual Reviews
Citation
Peterman, Emma L, Ploessl, Deon S and Galloway, Kate E. 2024. "Accelerating Diverse Cell-Based Therapies Through Scalable Design." Annual Review of Chemical and Biomolecular Engineering, 15 (1).
Version
Final published version
Abstract
Augmenting cells with novel, genetically encoded functions will support therapies that expand beyond natural capacity for immune surveillance and tissue regeneration. However, engineering cells at scale with transgenic cargoes remains a challenge in realizing the potential of cell-based therapies. In this review, we introduce a range of applications for engineering primary cells and stem cells for cell-based therapies. We highlight tools and advances that have launched mammalian cell engineering from bioproduction to precision editing of therapeutically relevant cells. Additionally, we examine how transgenesis methods and genetic cargo designs can be tailored for performance. Altogether, we offer a vision for accelerating the translation of innovative cell-based therapies by harnessing diverse cell types, integrating the expanding array of synthetic biology tools, and building cellular tools through advanced genome writing techniques.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1146/annurev-chembioeng-100722-121610
