MARIS: Method for Analyzing RNA following Intracellular Sorting
Name
Hrvatin-2014-MARIS_ Method for An.pdf
Size
878.26 KB
Format
Adobe PDF
Checksum (MD5)
f195cf69923b1b5ca86d18ec08c5fe68
Author(s)
Hrvatin, Sinisa
Deng, Francis
O'Donnell, Charles William
Gifford, David K.
Melton, Douglas A.
Date Issued
March 2014
Journal
PLoS ONE
Publisher
Public Library of Science
Citation
Hrvatin, Sinisa, Francis Deng, Charles W. O’Donnell, David K. Gifford, and Douglas A. Melton. “MARIS: Method for Analyzing RNA Following Intracellular Sorting.” Edited by Katriina Aalto-Setala. PLoS ONE 9, no. 3 (March 3, 2014): e89459.
Version
Final published version
Abstract
Transcriptional profiling is a key technique in the study of cell biology that is limited by the availability of reagents to uniquely identify specific cell types and isolate high quality RNA from them. We report a Method for Analyzing RNA following Intracellular Sorting (MARIS) that generates high quality RNA for transcriptome profiling following cellular fixation, intracellular immunofluorescent staining and FACS. MARIS can therefore be used to isolate high quality RNA from many otherwise inaccessible cell types simply based on immunofluorescent tagging of unique intracellular proteins. As proof of principle, we isolate RNA from sorted human embryonic stem cell-derived insulin-expressing cells as well as adult human β cells. MARIS is a basic molecular biology technique that could be used across several biological disciplines.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1371/journal.pone.0089459
