Reducing Cancer Cell Adhesion using Microtextured Surfaces
Name
Small - 2023 - McCue - Reducing Cancer Cell Adhesion using Microtextured Surfaces.pdf
Description
Published version
Size
3.2 MB
Format
Adobe PDF
Checksum (MD5)
e6f9c17e3b9fc66cb090cdad0cf7201d
Author(s)
McCue, Caroline
Atari, Adel
Parks, Sean
Tseng, Yuen‐Yi
Varanasi, Kripa K.
Date Issued
August 9, 2023
Journal
Small
Publisher
Wiley
Citation
C. McCue, A. Atari, S. Parks, Y.-Y. Tseng, K. K. Varanasi, Reducing Cancer Cell Adhesion using Microtextured Surfaces. Small 2023, 19, 2302401.
Version
Final published version
Abstract
For the past century, trypsin has been the primary method of cell dissociation, largely without any major changes to the process. Enzymatic cell detachment strategies for large‐scale cell culturing processes are popular but can be labor‐intensive, potentially lead to the accumulation of genetic mutations, and produce large quantities of liquid waste. Therefore, engineering surfaces to lower cell adhesion strength could enable the next generation of cell culture surfaces for delicate primary cells and automated, high‐throughput workflows. In this study, a process for creating microtextured polystyrene (PS) surfaces to measure the impact of microposts on the adhesion strength of cells is developed. Cell viability and proliferation assays show comparable results in two cancer cell lines between micropost surfaces and standard cell culture vessels. However, cell image analysis on microposts reveals that cell area decreases by half, and leads to an average twofold increase in cell length per area. Using a microfluidic‐based method up to a seven times greater percentage of cells are removed from micropost surfaces than the flat control surfaces. These results show that micropost surfaces enable decreased cell adhesion strength while maintaining similar cell viabilities and proliferation as compared to flat PS surfaces.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/smll.202302401
