Microfluidic label-free selection of mesenchymal stem cell subpopulation during culture expansion extends the chondrogenic potential
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c7lc01005b.pdf
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Author(s)
Yin, Lu
Wu, Yingnan
Yang, Zheng
Tee, Ching Ann
Denslin, Vinitha
Lai, Zhangxing
Lim, Chwee Teck
Lee, Eng Hin
Han, Jongyoon
Date Issued
February 2018
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry (RSC)
Citation
Yin, Lu et al. “Microfluidic Label-Free Selection of Mesenchymal Stem Cell Subpopulation During Culture Expansion Extends the Chondrogenic Potential in Vitro.” Lab on a Chip 18, 6 (2018): 878–889 © 2018 Royal Society of Chemistry
Version
Final published version
Abstract
Mesenchymal stem cells (MSCs) have been shown as potential candidates for cell-based therapies for a diverse range of tissue regenerative applications. Therapeutic use of MSCs usually requires culture expansion, which increases the heterogeneity of MSCs in vitro, thus affecting the potency of the MSCs for more specific indications. The capacity for identifying and isolating special subsets of MSCs for treatment of specific diseases therefore holds great clinical significance. An important therapeutic application of MSC is for the regeneration of cartilage tissue. We and others have previously developed label-free microfluidic means to isolate subpopulations of culture expanded MSCs based on distinct biophysical characteristics. Here we utilize a spiral micro-channel device to separate culture expanded MSCs into five subgroups according to cell size, and study their proliferation and chondrogenesis at early, middle and late passages. Results show that in all passages, the medium-size subpopulation (cell size of 17-21 μm), compared to other subpopulations, displays significantly higher proliferation rate and chondrogenic capacity in terms of cartilage extracellular matrix formation. Also, the small cell subpopulation (average cell size of 11-12 μm) shows lower viability, and large cell subpopulation (average cell size 23-25 μm) expresses higher level of senescence-associated β-galactosidase. Finally, we show that repeated microfluidic exclusion of MSCs larger than 21 μm and smaller than 17 μm at every passage during continuous culture expansion result in selected MSCs with faster proliferation and better chondrogenic potential as compared to MSC derived from conventional expansion approach. This study demonstrates the significant merit and utility of size-based cell selection for the application of MSCs in cartilage regeneration.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Creative Commons Attribution-NonCommercial 3.0 Unported
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DOI of Published Version
https://doi.org/10.1039/C7LC01005B
