Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology
Name
nihms-1621502.pdf
Description
Accepted version
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4.19 MB
Format
Adobe PDF
Checksum (MD5)
86f2512ce4db4a0c47c5872734accfee
Author(s)
Bucur, Octavian
Fu, Feifei
Calderon, Mike
Mylvaganam, Geetha H
Ly, Ngoc L
Day, Jimmy
Watkin, Simon
Walker, Bruce D
Boyden, Edward S
Zhao, Yongxin
Date Issued
2020
Journal
Nature Protocols
Publisher
Springer Science and Business Media LLC
Citation
Bucur, Octavian, Fu, Feifei, Calderon, Mike, Mylvaganam, Geetha H, Ly, Ngoc L et al. 2020. "Nanoscale imaging of clinical specimens using conventional and rapid-expansion pathology." Nature Protocols, 15 (5).
Version
Author's final manuscript
Abstract
© 2020, The Author(s), under exclusive licence to Springer Nature Limited. In pathology, microscopy is an important tool for the analysis of human tissues, both for the scientific study of disease states and for diagnosis. However, the microscopes commonly used in pathology are limited in resolution by diffraction. Recently, we discovered that it was possible, through a chemical process, to isotropically expand preserved cells and tissues by 4–5× in linear dimension. We call this process expansion microscopy (ExM). ExM enables nanoscale resolution imaging on conventional microscopes. Here we describe protocols for the simple and effective physical expansion of a variety of human tissues and clinical specimens, including paraffin-embedded, fresh frozen and chemically stained human tissues. These protocols require only inexpensive, commercially available reagents and hardware commonly found in a routine pathology laboratory. Our protocols are written for researchers and pathologists experienced in conventional fluorescence microscopy. The conventional protocol, expansion pathology, can be completed in ~1 d with immunostained tissue sections and 2 d with unstained specimens. We also include a new, fast variant, rapid expansion pathology, that can be performed on <5-µm-thick tissue sections, taking <4 h with immunostained tissue sections and <8 h with unstained specimens.
MIT Department
Massachusetts Institute of Technology. Media Laboratory
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biological Engineering
McGovern Institute for Brain Research at MIT
Koch Institute for Integrative Cancer Research at MIT
Massachusetts Institute of Technology. Center for Neurobiological Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/S41596-020-0300-1
