Embryoid body size-mediated differential endodermal and mesodermal differentiation using polyethylene glycol (PEG) microwell array
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Author(s)
Bae, Hojae
Hwang, Yu-Shik
Kwon, Il Keun
Sadr, Nasser
Manoucheri, Sam
Edalat, Faramarz
Kim, Keekyoung
Khademhosseini, Alireza
Cha, Jae Min
Kim, Sang Bok
Date Issued
March 2015
Journal
Macromolecular Research
Publisher
The Polymer Society of Korea
Citation
Cha, Jae Min, Hojae Bae, Nasser Sadr, Sam Manoucheri, Faramarz Edalat, Keekyoung Kim, Sang Bok Kim, Il Keun Kwon, Yu-Shik Hwang, and Ali Khademhosseini. “Embryoid Body Size-Mediated Differential Endodermal and Mesodermal Differentiation Using Polyethylene Glycol (PEG) Microwell Array.” Macromolecular Research 23, no. 3 (March 2015): 245–255.
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Author's final manuscript
Abstract
Embryoid bodies have a number of similarities with cells in gastrulation, which provides useful biological information about embryonic stem cell differentiation. Extensive research has been done to study the control of embryoid body-mediated embryonic stem cell differentiation in various research fields. Recently, microengineering technology has been used to control the size of embryoid bodies and to direct lineage specific differentiation of embryonic stem cells. However, the underlying biology of developmental events in the embryoid bodies of different sizes has not been well elucidated. In this study, embryoid bodies with different sizes were generated within microfabricated PEG microwell arrays, and a series of gene and molecular expressions related to early developmental events was investigated to further elucidate the size-mediated differentiation. The gene and molecular expression profile suggested preferential visceral endoderm formation in 450 μm embryoid bodies and preferential lateral plate mesoderm formation in 150 μm embryoid bodies. These aggregates resulted in higher cardiac differentiation in 450 μm embryoid bodies and higher endothelial differentiation in 150 μm embryoid bodies, respectively. Our findings may provide further insight for understanding embryoid body size-mediated developmental progress.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1007/s13233-015-3034-0
