Q&A: Expansion microscopy
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s12915-017-0393-3.pdf
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1.71 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Gao, Ruixuan
Asano, Shoh M
Boyden, Edward
Date Issued
June 2017
Journal
BMC Biology
Publisher
Biomed Central Ltd
Citation
Gao, Ruixuan et al. “Q&A: Expansion Microscopy.” BMC Biology 15, 1 (June 2017) © 2017 The Author(s)
Version
Final published version
Abstract
Expansion microscopy (ExM) is a recently invented technology that uses swellable charged polymers, synthesized densely and with appropriate topology throughout a preserved biological specimen, to physically magnify the specimen 100-fold in volume, or more, in an isotropic fashion. ExM enables nanoscale resolution imaging of preserved samples on inexpensive, fast, conventional microscopes. How does ExM work? How good is its performance? How do you get going on using it? In this Q & A, we provide the answers to these and other questions about this new and rapidly spreading toolbox.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International
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DOI of Published Version
https://doi.org/10.1186/s12915-017-0393-3
