Precision Calcium Imaging of Dense Neural Populations via a Cell-Body-Targeted Calcium Indicator
Name
nihms-1599109.pdf
Description
Accepted version
Size
3.32 MB
Format
Unknown
Checksum (MD5)
8dbd20476bc8bb2c2621b09b2ebadc6a
Author(s)
Shemesh, Or A.
Linghu, Changyang
Piatkevich, Kiryl D.
Goodwin, Daniel
Celiker, Orhan Tunc
Gritton, Howard J.
Romano, Michael F.
Gao, Ruixuan
Yu, Chih-Chieh (Jay)
Tseng, Hua-An
Date Issued
August 2020
Journal
Neuron
Publisher
Elsevier BV
Version
Author's final manuscript
Abstract
© 2020 Elsevier Inc. One-photon fluorescent imaging of calcium signals can capture the activity of hundreds of neurons across large fields of view but suffers from crosstalk from neuropil. Shemesh et al. engineer cell-body-targeted variants of fluorescent calcium indicators and show in mice and zebrafish that artifactual spikes and correlations are greatly reduced.
MIT Department
Massachusetts Institute of Technology. Media Laboratory
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Center for Neurobiological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Picower Institute for Learning and Memory
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.neuron.2020.05.029
