Parallel gene synthesis in a microfluidic device
Name
Kong-2007-Parallel gene synthesis in a microfluidic device.pdf
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2.09 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Kong, David S.
Carr, Peter A., Sr.
Chen, Lu
Zhang, Shuguang
Jacobson, Joseph
Date Issued
April 2007
Journal
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Citation
Kong, D. S. et al. “Parallel Gene Synthesis in a Microfluidic Device.” Nucleic Acids Research 35.8 (2007): e61–e61. Web. 1 June 2012.
Version
Final published version
Abstract
The ability to synthesize custom de novo DNA constructs rapidly, accurately and inexpensively is highly desired by researchers, as synthetic genes and longer DNA constructs are enabling to numerous powerful applications in both traditional molecular biology and the emerging field of synthetic biology. However, the current cost of de novo synthesis—driven largely by reagent and handling costs—is a significant barrier to the widespread availability of such technology. In this work, we demonstrate, to our knowledge, the first gene synthesis in a microfluidic environment. The use of microfluidic technology greatly reduces reaction volumes and the corresponding reagent and handling costs. Additionally, microfluidic technology enables large numbers of complex reactions to be performed in parallel. Here, we report the fabrication of a multi-chamber microfluidic device and its use in carrying out the syntheses of several DNA constructs. Genes up to 1 kb in length were synthesized in parallel at minute starting oligonucleotide concentrations (10–25 nM) in four 500 nl reactors. Such volumes are one to two orders of magnitude lower than those utilized in conventional gene synthesis. The identity of all target genes was verified by sequencing, and the resultant error rate was determined to be 1 per 560 bases.
MIT Department
Lincoln Laboratory
Massachusetts Institute of Technology. Center for Biomedical Engineering
Massachusetts Institute of Technology. Center for Bits and Atoms
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Media Laboratory
Terms of Use
Creative Commons Attribution Non-Commercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1093/nar/gkm121
