An Ultra-Sensitive Step-Function Opsin for Minimally Invasive Optogenetic Stimulation in Mice and Macaques
Name
nihms-1582991.pdf
Description
Accepted version
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1.22 MB
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Author(s)
Gong, Xin
Mendoza-Halliday, Diego
Ting, Jonathan T.
Kaiser, Tobias
Sun, Xuyun
Bastos, André M.
Wimmer, Ralf D.
Guo, Baolin
Chen, Qian
Zhou, Yang
Date Issued
July 2020
Journal
Neuron
Publisher
Elsevier BV
Citation
© 2020 Elsevier Inc.
Version
Author's final manuscript
Abstract
Optogenetics is among the most widely employed techniques to manipulate neuronal activity. However, a major drawback is the need for invasive implantation of optical fibers. To develop a minimally invasive optogenetic method that overcomes this challenge, we engineered a new step-function opsin with ultra-high light sensitivity (SOUL). We show that SOUL can activate neurons located in deep mouse brain regions via transcranial optical stimulation and elicit behavioral changes in SOUL knock-in mice. Moreover, SOUL can be used to modulate neuronal spiking and induce oscillations reversibly in macaque cortex via optical stimulation from outside the dura. By enabling external light delivery, our new opsin offers a minimally invasive tool for manipulating neuronal activity in rodent and primate models with fewer limitations on the depth and size of target brain regions and may further facilitate the development of minimally invasive optogenetic tools for the treatment of neurological disorders.
MIT Department
McGovern Institute for Brain Research at MIT
Picower Institute for Learning and Memory
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1016/j.neuron.2020.03.032
