A Comprehensive Map of Insulator Elements for the Drosophila Genome
Name
Negre-2010-A Comprehensive Map.pdf
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1.89 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
White, Kevin P.
Henikoff, Steven
Stein, Lincoln
White, Robert A. H.
Russell, Steven
Ahmad, Kami
Feng, Xin
Henikoff, Jorja G.
Morrison, Carolyn A.
Shah, Parantu K.
Date Issued
January 2010
Journal
PLoS Genetics
Publisher
Public Library of Science
Citation
Nègre, Nicolas et al. “A Comprehensive Map of Insulator Elements for the Drosophila Genome.” PLoS Genet 6.1 (2010): e1000814. © 2010 Nègre et al.
Version
Final published version
Abstract
Insulators are DNA sequences that control the interactions among genomic regulatory elements and act as chromatin boundaries. A thorough understanding of their location and function is necessary to address the complexities of metazoan gene regulation. We studied by ChIP–chip the genome-wide binding sites of 6 insulator-associated proteins—dCTCF, CP190, BEAF-32, Su(Hw), Mod(mdg4), and GAF—to obtain the first comprehensive map of insulator elements in Drosophila embryos. We identify over 14,000 putative insulators, including all classically defined insulators. We find two major classes of insulators defined by dCTCF/CP190/BEAF-32 and Su(Hw), respectively. Distributional analyses of insulators revealed that particular sub-classes of insulator elements are excluded between cis-regulatory elements and their target promoters; divide differentially expressed, alternative, and divergent promoters; act as chromatin boundaries; are associated with chromosomal breakpoints among species; and are embedded within active chromatin domains. Together, these results provide a map demarcating the boundaries of gene regulatory units and a framework for understanding insulator function during the development and evolution of Drosophila.
MIT Department
Massachusetts Institute of Technology. Computer Science and Artificial Intelligence Laboratory
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
http://dx.doi.org/10.1371/journal.pgen.1000814
