Enhanced multiplex genome engineering through co-operative oligonucleotide co-selection
Name
Carr-2012-Enhanced multiplex g.pdf
Size
3.94 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Carr, Peter A., Sr.
Wang, Harris H.
Sterling, Bram Henry
Isaacs, Farren J.
Lajoie, Marc J.
Xu, George Jing
Church, George M.
Jacobson, Joseph
Date Issued
May 2012
Journal
Nucleic Acids Research
Publisher
Oxford University Press (OUP)
Citation
Carr, P. A. et al. “Enhanced Multiplex Genome Engineering Through Co-operative Oligonucleotide Co-selection.” Nucleic Acids Research (2012).
Version
Final published version
Abstract
Genome-scale engineering of living organisms requires precise and economical methods to efficiently modify many loci within chromosomes. One such example is the directed integration of chemically synthesized single-stranded deoxyribonucleic acid (oligonucleotides) into the chromosome of Escherichia coli during replication. Herein, we present a general co-selection strategy in multiplex genome engineering that yields highly modified cells. We demonstrate that disparate sites throughout the genome can be easily modified simultaneously by leveraging selectable markers within 500 kb of the target sites. We apply this technique to the modification of 80 sites in the E. coli genome.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Center for Bits and Atoms
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
Terms of Use
Creative Commons Attribution Non-Commercial
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1093/nar/gks455
