Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip
Name
s41698-019-0102-5.pdf
Description
Published version
Size
3.95 MB
Format
Adobe PDF
Checksum (MD5)
12515f2c53c73b9b680c53cc42c5b2b9
Author(s)
Khoo, Bee Luan
Shang, Menglin
Ng, Chin Hin
Lim, Chwee-Teck
Chng, Wee Joo
Han, Jongyoon
Date Issued
December 2019
Journal
npj Precision Oncology
Publisher
Springer
Citation
Khoo, Bee Luan et al. "Liquid biopsy for minimal residual disease detection in leukemia using a portable blast cell biochip." npj Precision Oncology 3, 1 (December 2019): 30
Version
Final published version
Abstract
Long-term management for leukemia is challenging due to the painful and invasive procedure of bone marrow (BM) biopsy. At present, non-invasive liquid (blood) biopsy is not utilized for leukemia, due to lower counts of leukemia blast cells in the blood. Here, we described a robust system for the simultaneous detection and enrichment of rare blast cells. Enrichment of blast cells was achieved from blood with a one-step microfluidic blast cell biochip (BCB) sorting system, without specific targeting of proteins by antibodies. Non-target cells encountered a differential net force as compared to stiffer blast cells and were removed. The efficiency of the BCB promotes high detection sensitivity (1 in 106 cells) even from patients with minimal residual disease. The procedure was validated using actual blast cells from patients with various types of leukemia. Outcomes were compared to current evaluation standards, such as flow cytometry, using BM aspirates. Blast cell detection efficiency was higher in 55.6% of the patients using the BCB as compared to flow cytometry, despite the lower concentrations of blast cells in liquid biopsy. These studies promote early-stage detection and routine monitoring for minimal residual disease in patients.
MIT Department
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Department of Biological Engineering
Singapore-MIT Alliance in Research and Technology (SMART)
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41698-019-0102-5
