Modular glycosphere assays for high-throughput functional characterization of influenza viruses
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1472-6750-13-34.pdf
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Author(s)
Hobbie, Sven N.
Viswanathan, Karthik
Bachelet, Ido
Aich, Udayanath
Subramanian, Vidya
Raman, Rahul
Sasisekharan, Ram
Shriver, Zachary H.
Date Issued
April 2013
Journal
BMC Biotechnology
Publisher
BioMed Central Ltd
Citation
Hobbie, Sven N, Karthik Viswanathan, Ido Bachelet, Udayanath Aich, Zachary Shriver, Vidya Subramanian, Rahul Raman, and Ram Sasisekharan. “Modular glycosphere assays for high-throughput functional characterization of influenza viruses.” BMC Biotechnology 13, no. 1 (2013): 34
Version
Final published version
Abstract
Background:
The ongoing global efforts to control influenza epidemics and pandemics require high-throughput technologies to detect, quantify, and functionally characterize viral isolates. The 2009 influenza pandemic as well as the recent in-vitro selection of highly transmissible H5N1 variants have only increased existing concerns about emerging influenza strains with significantly enhanced human-to-human transmissibility. High-affinity binding of the virus hemagglutinin to human receptor glycans is a highly sensitive and stringent indicator of host adaptation and virus transmissibility. The surveillance of receptor-binding characteristics can therefore provide a strong additional indicator for the relative hazard imposed by circulating and newly emerging influenza strains.
Results:
Streptavidin-coated microspheres were coated with selected biotinylated glycans to mimic either human or avian influenza host-cell receptors. Such glycospheres were used to selectively capture influenza virus of diverse subtypes from a variety of samples. Bound virus was then detected by fluorescently labelled antibodies and analyzed by quantitative flow cytometry. Recombinant hemagglutinin, inactivated virus, and influenza virions were captured and analyzed with regards to receptor specificity over a wide range of analyte concentration. High-throughput analyses of influenza virus produced dose-response curves that allow for functional assessment of relative receptor affinity and thus transmissibility.
Conclusions:
Modular glycosphere assays for high-throughput functional characterization of influenza viruses introduce an important tool to augment the surveillance of clinical and veterinarian influenza isolates with regards to receptor specificity, host adaptation, and virus transmissibility.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
Massachusetts Institute of Technology. School of Engineering
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1186/1472-6750-13-34
