Isotope tracing in health and disease
Name
1-s2.0-S0958166922000738-main.pdf
Description
Published version
Size
3.62 MB
Format
Adobe PDF
Checksum (MD5)
7ff0fbe2fff6ba1ad2eebeaa602d52b4
Author(s)
Dong, Wentao
Rawat, Eshaan S
Stephanopoulos, Gregory
Abu-Remaileh, Monther
Date Issued
August 2022
Journal
Current Opinion in Biotechnology
Publisher
Elsevier BV
Citation
Dong, Wentao, Rawat, Eshaan S, Stephanopoulos, Gregory and Abu-Remaileh, Monther. 2022. "Isotope tracing in health and disease." Current Opinion in Biotechnology, 76.
Version
Final published version
Abstract
Biochemical characterization of metabolism provides molecular insights for understanding biology in health and disease. Over the past decades, metabolic perturbations have been implicated in cancer, neurodegeneration, and diabetes, among others. Isotope tracing is a technique that allows tracking of labeled atoms within metabolites through biochemical reactions. This technique has become an integral component of the contemporary metabolic research. Isotope tracing measures substrate contribution to downstream metabolites and indicates its utilization in cellular metabolic networks. In addition, isotopic labeling data are necessary for quantitative metabolic flux analysis. Here, we review recent work utilizing metabolic tracing to study health and disease, and highlight its application to interrogate subcellular, intercellular, and in vivo metabolism. We further discuss the current challenges and opportunities to expand the utility of isotope tracing to new research areas.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.copbio.2022.102739
