Peritoneal fluid cytokines related to endometriosis in patients evaluated for infertility
Author(s)
Jørgensen, Hilde
Hill, Abby
Beste, Michael T.
Kumar, Manu Prajapati
Chiswick, Evan
Fedorcsak, Peter
Isaacson, Keith B.
Lauffenburger, Douglas A.
Griffith, Linda G.
Qvigstad, Erik
Date Issued
April 2017
Journal
Fertility and Sterility
Publisher
Elsevier BV
Citation
Jørgensen, Hilde et al. "Peritoneal fluid cytokines related to endometriosis in patients evaluated for infertility." Fertility and Sterility, 107, 5 (May 2017): 1191-1199.e2
Version
Original manuscript
Abstract
Objective: Our aim was to characterize peritoneal cytokine profiles in patients with infertility, with and without endometriosis, to illuminate potential differences in immune profiles that may reflect mechanistic differences between these two patient populations. Design: Cross-sectional study. Setting: University hospital and research center. Patient(s): Women undergoing laparoscopy for infertility investigation (n = 107). Intervention(s): Peritoneal fluid was collected during surgery. Clinical characteristics were registered preoperatively. Main Outcome Measure(s): We determined the concentration of 48 different cytokines from the peritoneal fluid with multiplex immunoassays. Associations between cytokines and clinical findings were assessed with logistic regression and partial least squares discriminant analyses (PLS-DA). Result(s): Concentrations of SCGF-β, IL-8, HGF, and MCP-1 were significantly higher, while IL-13 was significantly lower in the endometriosis group compared with the group without endometriosis. Multiple stepwise logistic regression identified a combination of SCGF-β, IL-13, and G-CSF concentrations that predicted the presence of endometriosis with 86% sensitivity and 67% specificity. PLS-DA identified a class of 11 cytokines (SCGF-β, HGF, IL-13, MCP-1, CTACK, MCP-3, M-CSF, LIF, IL-5, IL-9, and IFN-a2) that were more characteristic of endometriosis than nonendometriosis patients. Conclusion(s): By combining univariate and multivariate analyses, profiles of cytokines more likely to be enriched or depleted in infertility patients with endometriosis compared with those without endometriosis were identified. These findings may inform future analyses of pathophysiological mechanisms of endometriosis in infertile patients, including dysregulated Th1/Th2 response and mobilization and proliferation of hematopoietic stem cells. Keyword: Cytokine; endometriosis; infertility; peritoneal fluid; PLS-DA
MIT Department
Massachusetts Institute of Technology. Center for Gynepathology Research
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/j.fertnstert.2017.03.013
