Polyimide Electrode-Based Electrical Stimulation Impedes Early Stage Muscle Graft Regeneration
Name
fneur-10-00252.pdf
Description
Published version
Size
2.21 MB
Format
Adobe PDF
Checksum (MD5)
f1b71f5386857c30d3f0817f60627a0d
Author(s)
Srinivasan, Shriya Sruthi
Vyas, Keval N
McAvoy, Malia
Calvaresi, Peter
Khan, Omar Fizal
Langer, Robert S
Anderson, Daniel Griffith
Herr, Hugh
Date Issued
March 2019
Journal
Frontiers in Neurology
Publisher
Frontiers Media SA
Citation
Srinivasan, Shriya et al. "Polyimide Electrode-Based Electrical Stimulation Impedes Early Stage Muscle Graft Regeneration." Frontiers in Neurology 10 (March 2019): 252 © 2019 The Authors
Version
Final published version
Abstract
Given the increasing use of regenerative free muscle flaps for various reconstructive procedures and neuroprosthetic applications, there is great interest and value in their enhanced regeneration, revascularization, and reinnervation for improved functional recovery. Here, we implant polyimide-based mircroelectrodes on free flap grafts and perform electrical stimulation for 6 weeks in a murine model. Using electrophysiological and histological assessments, we compare outcomes of stimulated grafts with unstimulated control grafts. We find delayed reinnervation and abnormal electromyographic (EMG) signals, with significantly more polyphasia, lower compound muscle action potentials and higher fatigability in stimulated animals. These metrics are suggestive of myopathy in the free flap grafts stimulated with the electrode. Additionally, active inflammatory processes and partial necrosis are observed in grafts stimulated with the implanted electrode. The results suggest that under this treatment protocol, implanted epimysial electrodes and electrical stimulation to deinnervated, and devascularized flaps during the early recovery phase may be detrimental to regeneration. Future work should determine the optimal implantation and stimulation window for accelerating free muscle graft regeneration.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3389/fneur.2019.00252
