Optogenetic feedback control of neural activity
Name
Newman-2015-Optogenetic feedback.pdf
Size
5.92 MB
Format
Adobe PDF
Checksum (MD5)
7549f702b4e0f1902c7f963dc214e7b3
Author(s)
Fong, Ming-fai
Newman, Jonathan P.
Millard, Daniel C.
Whitmire, Clarissa J.
Stanley, Garrett B.
Potter, Steve M.
Date Issued
July 2015
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
Newman, Jonathan P, Ming-fai Fong, Daniel C Millard, Clarissa J Whitmire, Garrett B Stanley, and Steve M Potter. “Optogenetic Feedback Control of Neural Activity.” eLife 4 (July 3, 2015).
Version
Final published version
Abstract
Optogenetic techniques enable precise excitation and inhibition of firing in specified neuronal populations and artifact-free recording of firing activity. Several studies have suggested that optical stimulation provides the precision and dynamic range requisite for closed-loop neuronal control, but no approach yet permits feedback control of neuronal firing. Here we present the ‘optoclamp’, a feedback control technology that provides continuous, real-time adjustments of bidirectional optical stimulation in order to lock spiking activity at specified targets over timescales ranging from seconds to days. We demonstrate how this system can be used to decouple neuronal firing levels from ongoing changes in network excitability due to multi-hour periods of glutamatergic or GABAergic neurotransmission blockade in vitro as well as impinging vibrissal sensory drive in vivo. This technology enables continuous, precise optical control of firing in neuronal populations in order to disentangle causally related variables of circuit activation in a physiologically and ethologically relevant manner.
MIT Department
Picower Institute for Learning and Memory
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.7554/eLife.07192
