Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
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Author(s)
Levy, Oren
Anandakumaran, Priya
Ngai, Jessica
Karnik, Rohit
Karp, Jeffrey Michael
Date Issued
October 2013
Journal
Journal of Visualized Experiments
Publisher
MyJove Corporation
Citation
Levy, Oren et al. “Systematic Analysis of In Vitro Cell Rolling Using a Multi-Well Plate Microfluidic System.” Journal of Visualized Experiments 80 (October 2013): e50866 © 2013 Journal of Visualized Experiments.
Version
Final published version
Abstract
A major challenge for cell-based therapy is the inability to systemically target a large quantity of viable cells with high efficiency to tissues of interest following intravenous or intraarterial infusion. Consequently, increasing cell homing is currently studied as a strategy to improve cell therapy. Cell rolling on the vascular endothelium is an important step in the process of cell homing and can be probed in-vitro using a parallel plate flow chamber (PPFC). However, this is an extremely tedious, low throughput assay, with poorly controlled flow conditions. Instead, we used a multi-well plate microfluidic system that enables study of cellular rolling properties in a higher throughput under precisely controlled, physiologically relevant shear flow1,2. In this paper, we show how the rolling properties of HL-60 (human promyelocytic leukemia) cells on P-and E-selectin-coated surfaces as well as on cell monolayer-coated surfaces can be readily examined. To better simulate inflammatory conditions, the microfluidic channel surface was coated with endothelial cells (ECs), which were then activated with tumor necrosis factor-a (TNF-a), significantly increasing interactions with HL-60 cells under dynamic conditions. The enhanced throughput and integrated multi-parameter software analysis platform, that permits rapid analysis of parameters such as rolling velocities and rolling path, are important advantages for assessing cell rolling properties in-vitro. Allowing rapid and accurate analysis of engineering approaches designed to impact cell rolling and homing, this platform may help advance exogenous cell-based therapy.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Mechanical Engineering
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DOI of Published Version
https://doi.org/10.3791/50866
