RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin
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lander 1.pdf
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213.42 KB
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222f0eec31a3228b1503edb11fd17bc9
Author(s)
Engreitz, Jesse Michael
Lander, Eric Steven
Guttman, Mitchell
Date Issued
2015
Journal
Nuclear Bodies and Noncoding RNAs
Publisher
Springer New York
Citation
Engreitz, Jesse, Lander, Eric S., and Guttman, Mitchell. “RNA Antisense Purification (RAP) for Mapping RNA Interactions with Chromatin.” Nuclear Bodies and Noncoding RNAs (December 5, 2014): 183–197.
Version
Author's final manuscript
Abstract
RNA-centric biochemical purification is a general approach for studying the functions and mechanisms of noncoding RNAs. Here, we describe the experimental procedures for RNA antisense purification (RAP), a method for selective purification of endogenous RNA complexes from cell extracts that enables mapping of RNA interactions with chromatin. In RAP, the user cross-links cells to fix endogenous RNA complexes and purifies these complexes through hybrid capture with biotinylated antisense oligos. DNA loci that interact with the target RNA are identified using high-throughput DNA sequencing.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1007/978-1-4939-2253-6_11
