Three-dimensional manipulation of single cells using surface acoustic waves
Name
Guo-2016-Three-dimensional ma.pdf
Size
1.13 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Guo, Feng
Mao, Zhangming
Chen, Yuchao
Xie, Zhiwei
Lata, James P.
Li, Peng
Ren, Liqiang
Liu, Jiayang
Yang, Jian
Dao, Ming
Date Issued
February 2016
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
National Academy of Sciences (U.S.)
Citation
Guo, Feng et al. “Three-Dimensional Manipulation of Single Cells Using Surface Acoustic Waves.” Proceedings of the National Academy of Sciences 113.6 (2016): 1522–1527.
Version
Final published version
Abstract
The ability of surface acoustic waves to trap and manipulate micrometer-scale particles and biological cells has led to many applications involving “acoustic tweezers” in biology, chemistry, engineering, and medicine. Here, we present 3D acoustic tweezers, which use surface acoustic waves to create 3D trapping nodes for the capture and manipulation of microparticles and cells along three mutually orthogonal axes. In this method, we use standing-wave phase shifts to move particles or cells in-plane, whereas the amplitude of acoustic vibrations is used to control particle motion along an orthogonal plane. We demonstrate, through controlled experiments guided by simulations, how acoustic vibrations result in micromanipulations in a microfluidic chamber by invoking physical principles that underlie the formation and regulation of complex, volumetric trapping nodes of particles and biological cells. We further show how 3D acoustic tweezers can be used to pick up, translate, and print single cells and cell assemblies to create 2D and 3D structures in a precise, noninvasive, label-free, and contact-free manner.
MIT Department
Massachusetts Institute of Technology. Center for Materials Science and Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1073/pnas.1524813113
