Speckle-field digital holographic microscopy
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Feld_Speckle-field digital.PDF
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Author(s)
Badizadegan, Kamran
Dasari, Ramachandra Rao
Yaqoob, Zahid
Choi, Wonshik
Feld, Michael S.
Park, YongKeun
Date Issued
July 2009
Journal
Optics Express
Publisher
Optical Society of America
Citation
Park,YongKeun et al. "Speckle-field digital holographic microscopy," Opt. Express 17, 12285-12292 (2009) ©2009 Optical Society of America.
Version
Author's final manuscript
Abstract
The use of coherent light in conventional holographic phase
microscopy (HPM) poses three major drawbacks: poor spatial resolution,
weak depth sectioning, and fixed pattern noise due to unwanted diffraction.
Here, we report a technique which can overcome these drawbacks, but
maintains the advantage of phase microscopy - high contrast live cell
imaging and 3D imaging. A speckle beam of a complex spatial pattern is
used for illumination to reduce fixed pattern noise and to improve optical
sectioning capability. By recording of the electric field of speckle, we
demonstrate high contrast 3D live cell imaging without the need for axial
scanning - neither objective lens nor sample stage. This technique has great
potential in studying biological samples with improved sensitivity,
resolution and optical sectioning capability.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Spectroscopy Laboratory
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Attribution-Noncommercial-Share Alike 3.0 Unported
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DOI of Published Version
http://dx.doi.org/10.1364/OE.17.012285
