Plasma membrane recovery kinetics of a microfluidic intracellular delivery platform
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Jensen_Plasma membrane.pdf
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Author(s)
Poceviciute, Roberta
Jackson, Emily L.
Cho, Nahyun
Mao, Shirley
Hartoularos, George C.
Jang, Derek Y.
Jhunjhunwala, Siddharth
Schoettle, Taylor
Sharei, Armon Reza
Eyerman, Alexandra T.
Date Issued
January 2014
Journal
Integrative Biology
Publisher
Royal Society of Chemistry
Citation
Sharei, Armon, Roberta Poceviciute, Emily L. Jackson, Nahyun Cho, Shirley Mao, George C. Hartoularos, Derek Y. Jang, et al. “Plasma Membrane Recovery Kinetics of a Microfluidic Intracellular Delivery Platform.” Integr. Biol. 6, no. 4 (2014): 470. © Royal Society of Chemistry
Version
Final published version
Abstract
Intracellular delivery of materials is a challenge in research and therapeutic applications. Physical methods of plasma membrane disruption have recently emerged as an approach to facilitate the delivery of a variety of macromolecules to a range of cell types. We use the microfluidic CellSqueeze delivery platform to examine the kinetics of plasma membrane recovery after disruption and its dependence on the calcium content of the surrounding buffer (recovery time ~5 min without calcium vs. ~30 s with calcium). Moreover, we illustrate that manipulation of the membrane repair kinetics can yield up to 5× improvement in delivery efficiency without significantly impacting cell viability. Membrane repair characteristics initially observed in HeLa cells are shown to translate to primary naïve murine T cells. Subsequent manipulation of membrane repair kinetics also enables the delivery of larger materials, such as antibodies, to these difficult to manipulate cells. This work provides insight into the membrane repair process in response to mechanical delivery and could potentially enable the development of improved delivery methods.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1039/c3ib40215k
