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Magnetic Retrieval of Encapsulated Beta Cell Transplants from Diabetic Mice Using Dual‐Function MRI Visible and Retrievable Microcapsules
Name
Delcassian magnetic 2019_author accepted manuscript.pdf
Description
Accepted version
Size
1.1 MB
Format
Adobe PDF
Checksum (MD5)
e0a4cb5359a3cbf67ba9fb3da76f2ac5
Author(s)
Delcassian, Derfogail
Luzhansky, Igor
Spanoudaki, Virginia
Bochenek, Matthew
McGladrigan, Collin
Nguyen, Amy
Norcross, Samuel
Zhu, Yuhan
Shan, Crystal Shuo
Hausser, Reed
Date Issued
2020
Journal
Advanced Materials
Publisher
Wiley
Version
Author's final manuscript
Abstract
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Encapsulated beta cell transplantation offers a potential cure for a subset of diabetic patients. Once transplanted, beta cell grafts can help to restore glycemic control; however, locating and retrieving cells in the event of graft failure may pose a surgical challenge. Here, a dual-function nanoparticle-loaded hydrogel microcapsule is developed that enables graft retrieval under an applied magnetic field. Additionally, this system facilitates graft localization via magnetic resonance imaging (MRI), and graft isolation from the immune system. Iron oxide nanoparticles encapsulated within alginate hydrogel capsules containing viable islets are transplanted and the in vitro and in vivo retrieval of capsules containing nanoparticles functionalized with various ligands are compared. Capsules containing islets co-encapsulated with COOH-coated nanoparticles restore normal glycemia in immunocompetent diabetic mice for at least 6 weeks, can be visualized using MRI, and are retrievable in a magnetic field. Application of a magnetic field for 90 s via a magnetically assisted retrieval device facilitates rapid retrieval of up to 94% (±3.1%) of the transplant volume 24 h after surgical implantation. This strategy aids monitoring of cell-capsule locations in vivo, facilitates graft removal at the end of the transplant lifetime, and may be applicable to many encapsulated cell transplant systems.
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
10.1002/ADMA.201904502
