An optical design enabling lightweight and large field-of-view head-mounted microscopes
Name
2021.09.03.458947v2.full.pdf
Description
Submitted version
Size
6.45 MB
Format
Adobe PDF
Checksum (MD5)
2ea5ba4c4dfbfeafb292eb2b94d4dafb
Author(s)
Scherrer, Joseph R
Lynch, Galen F
Zhang, Jie J
Fee, Michale S
Date Issued
March 16, 2023
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Scherrer, Joseph R, Lynch, Galen F, Zhang, Jie J and Fee, Michale S. 2023. "An optical design enabling lightweight and large field-of-view head-mounted microscopes." Nature Methods.
Version
Original manuscript
Abstract
Here we present a fluorescence microscope light path that enables imaging, during free behavior, of thousands of neurons in mice and hundreds of neurons in juvenile songbirds. The light path eliminates traditional illumination optics, allowing for head-mounted microscopes that have both a lower weight and a larger field of view (FOV) than previously possible. Using this light path, we designed two microscopes: one optimized for FOV (~4 mm FOV; 1.4 g), and the other optimized for weight (1.0 mm FOV; 1.0 g).
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/s41592-023-01806-1
