The impact of negative selection on thymocyte migration in the medulla
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Chakraborty_Impact of Negative.pdf
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4.43 MB
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Author(s)
Borgne, Marie Le
Ladi, Ena
Dzhagalov, Ivan
Herzmark, Paul
Liao, Ying Fang
Chakraborty, Arup K.
Robey, Ellen A.
Date Issued
June 2009
Journal
Nature Immunology
Publisher
Nature Publishing Group
Citation
Le Borgne, Marie et al. “The Impact of Negative Selection on Thymocyte Migration in the Medulla.” Nature Immunology 10.8 (2009): 823–830.
Version
Author's final manuscript
Abstract
Developing thymocytes are screened for self-reactivity before they exit the thymus, but how thymocytes scan the medulla for self antigens is unclear. Using two-photon microscopy, we observed that medullary thymocytes migrated rapidly and made frequent, transient contacts with dendritic cells. In the presence of a negative selecting ligand, thymocytes slowed, became confined to areas of approximately 30 mum in diameter and had increased contact with dendritic cells surrounding confinement zones. One third of polyclonal medullary thymocytes also showed confined, slower migration and may correspond to autoreactive thymocytes. Our data suggest that many autoreactive thymocytes do not undergo immediate arrest and death after encountering a negative selecting ligand but instead adopt an altered migration program while remaining in the medullary microenvironment.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
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Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1038/ni.1761
