Neoblast Specialization in Regeneration of the Planarian Schmidtea mediterranea
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Scimone-2014-Neoblast Specializat.pdf
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3.97 MB
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Author(s)
Scimone, M. Lucila
Lapan, Sylvain W.
Kravarik, Kellie Marie
Reddien, Peter
Scimone, M. Lucila
Lapan, Sylvain W.
Date Issued
July 2014
Journal
Stem Cell Reports
Publisher
Elsevier
Citation
Scimone, M. Lucila, Kellie M. Kravarik, Sylvain W. Lapan, and Peter W. Reddien. “Neoblast Specialization in Regeneration of the Planarian Schmidtea Mediterranea.” Stem Cell Reports 3, no. 2 (August 2014): 339–352.
Version
Final published version
Abstract
Planarians can regenerate any missing body part in a process requiring dividing cells called neoblasts. Historically, neoblasts have largely been considered a homogeneous stem cell population. Most studies, however, analyzed neoblasts at the population rather than the single-cell level, leaving the degree of heterogeneity in this population unresolved. We combined RNA sequencing of neoblasts from wounded planarians with expression screening and identified 33 transcription factors transcribed in specific differentiated cells and in small fractions of neoblasts during regeneration. Many neoblast subsets expressing distinct tissue-associated transcription factors were present, suggesting candidate specification into many lineages. Consistent with this possibility, klf, pax3/7, and FoxA were required for the differentiation of cintillo-expressing sensory neurons, dopamine-β-hydroxylase-expressing neurons, and the pharynx, respectively. Together, these results suggest that specification of cell fate for most-to-all regenerative lineages occurs within neoblasts, with regenerative cells of blastemas being generated from a highly heterogeneous collection of lineage-specified neoblasts.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution-Noncommercial-No deriviatives
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DOI of Published Version
https://doi.org/10.1016/j.stemcr.2014.06.001
